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Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
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JoVE Journal Cancer Research
Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

DOI:
10.3791/60996-v

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09:50 min

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April 10, 2020

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, ,
1Department of Molecular and Cellular Biochemistry,University of Kentucky, 2Markey Cancer Center,University of Kentucky

Chapters

  • 00:04Introduction
  • 00:54Fluorescently Labeling Cells with DiI(1,1 -Dioctadecyl-3,3,3 ,3 -Tetramethylindocarbocyanine Perchlorate)
  • 02:10Microinjecting Zebrafish Larvae
  • 04:37Setting up Drug Screen with Xenografted Zebrafish
  • 06:22Imaging Xenografted Zebrafish and Quantifying Fluorescence
  • 08:36Results: Xenografted Tumor Area
  • 09:22Conclusion

Summary

Automatic Translation

Automatic Translation

Zebrafish xenograft models allow for high-throughput drug screening and fluorescent imaging of human cancer cells in an in vivo microenvironment. We developed a workflow for large scale, automated drug screening on patient-derived leukemia samples in zebrafish using an automated fluorescence microscope equipped imaging unit.

Tags

Zebrafish XenograftDrug ScreeningPrimary Patient-derived TumorAutomated ImagingQuantificationHuman Cancer CellsDrug ResponseIn Vivo ReplicantCost-effectiveDii StainingCell ResuspensionAgarose PlateZebrafish DechlorinationMicroinjectionBorosilicate Glass Needle

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