Journal
/
/
斑马鱼原发性患者衍生肿瘤异种移植物的药物筛选
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
DOI:

09:50 min

April 10, 2020

, ,

Chapters

  • 00:04Introduction
  • 00:54Fluorescently Labeling Cells with DiI(1,1 -Dioctadecyl-3,3,3 ,3 -Tetramethylindocarbocyanine Perchlorate)
  • 02:10Microinjecting Zebrafish Larvae
  • 04:37Setting up Drug Screen with Xenografted Zebrafish
  • 06:22Imaging Xenografted Zebrafish and Quantifying Fluorescence
  • 08:36Results: Xenografted Tumor Area
  • 09:22Conclusion

Summary

Automatic Translation

斑马鱼异种移植模型允许在体内微环境中对人类癌细胞进行高通量药物筛选和荧光成像。我们使用配备自动化荧光显微镜的成像单元,为斑马鱼患者衍生白血病样本进行大规模的自动化药物筛查工作流程。

Related Videos

Read Article