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植物-微生物相互作用:丝状芽孢杆菌对马铃薯根系分泌物的转录反应
JoVE Journal
Biology
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JoVE Journal Biology
Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
DOI:

08:59 min

July 02, 2018

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Chapters

  • 00:04Title
  • 00:44Germinating Potato in Sterilized Conditions
  • 01:56Collecting Potato Root Exudates
  • 03:23Treatment and Sampling of Bacteria
  • 04:57RNA Isolation
  • 07:12Results: RNA Quality Check and Ratio Intensity Plot for Visualizing Differential Gene Expression
  • 08:30Conclusion

Summary

Automatic Translation

本议定书的目的是研究 endosphere 分离芽孢杆菌丝状对马铃薯根系分泌物的 transcriptomic 反应。这种方法有助于鉴定与植物微生物相互作用有关的重要细菌基因, 并原则上适用于其他菌和植物, 并作小的调整。

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