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Journal / Neuroscience / Visualization of Amyloid β Deposits in the…
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Neuroscience

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JoVE Journal Neuroscience
Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
 

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Article DOI: 10.3791/57645-v • 09:31 min • March 7th, 2019
March 7th, 2019 •
*1, *2, 1, 1, 1, 3, 4
1Department of Life and Medical Systems, Doshisha University, 2Bruker Daltonics K.K., 3The Brain Bank for Aging Research, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 4Graduate School of Brain Science, Doshisha University
* These authors contributed equally

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Summary
Automatic Translation

Molecular imaging with matrix-assisted laser desorption/ionization-based imaging mass spectrometry (MALDI-IMS) allows the simultaneous mapping of multiple analytes in biological samples. Here, we present a protocol for the detection and visualization of amyloid β protein on brain tissues of Alzheimer's disease and cerebral amyloid angiopathy samples using MALDI-IMS.

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Keywords: Amyloid Beta MALDI Imaging Mass Spectrometry Alzheimer's Disease Brain Tissue Autopsy Formic Acid Segmentation Maps Marker Proteins Peptides Plaque Subarachnoid Vasculature Cryostat ITO-coated Glass Slides Ethanol Wash Vacuum Drying
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