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JoVE Journal Cancer Research
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
 

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Article DOI: 10.3791/57653-v • 08:31 min • June 8th, 2018
June 8th, 2018 •
*1, *1, 2, 1, 1, 1, 1, 1, 1
1Division of Biology, Kansas State University, 2Bristol Medical School, Translational Health Sciences, University of Bristol
* These authors contributed equally

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Summary
Automatic Translation

Repair of double-strand DNA breaks is a dynamic process, requiring not only formation of repair complexes at the breaks, but also their resolution after the lesion is addressed. Here, we use immunofluorescence microscopy for transient and long-lasting double-stranded breaks as a tool to dissect this genome maintenance mechanism.

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Keywords: DNA Repair Double-strand DNA Breaks Immunofluorescence Microscopy DNA Repair Complex U2OS/DR-GFP Cells EDTA Trypsin DMEM Hydrogen Peroxide PBS PFA Non-ionic Detergent BSA Primary Antibodies DAPI
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