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Journal
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Immunology and Infection
/
新鲜冷冻胃肠标本的亚细胞分离
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
Please note that all translations are automatically generated.
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新鲜冷冻胃肠标本的亚细胞分离
DOI:
10.3791/57740-v
•
10:07 min
•
July 15, 2018
•
Irati Romero-Garmendia*
1
,
Amaia Jauregi-Miguel*
1
,
Izortze Santin
,
Jose Ramón Bilbao
,
Ainara Castellanos-Rubio
1
Department of Genetics, Physical Anthropology and Animal Physiology
,
University of the Basque Country (UPV-EHU), Biocruces Health Research Institute
,
2
Endocrinology and Diabetes Research Group, Biocruces Health Research Institute
,
UPV-EHU, CIBERDEM
Chapters
00:01
Title
01:29
Protocol
02:10
Biopsy homogenization
03:25
Cytoplasm isolation
04:21
Nuclear isolation
07:29
Representative Results
09:27
Conclusions
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以执行一个简单的细胞分离的胞质和核蛋白在人类新鲜和冰冻肠道活检。
Tags
Subcellular Fractionation
Gastrointestinal Specimens
Protein Localization
Intestinal Biopsy
Nuclear And Cytoplasmic Fractions
Homogenization
Detergent Lysis
Centrifugation
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