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映射代谢: 直接在组织中监测乳酸脱氢酶活性
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Biochemistry
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JoVE Journal Biochemistry
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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映射代谢: 直接在组织中监测乳酸脱氢酶活性

DOI:
10.3791/57760-v

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06:18 min

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June 21, 2018

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, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Chapters

  • 00:04Title
  • 00:31Generate Slides of Frozen Mouse Skin
  • 01:53Slides and Staining Solution Preparation
  • 02:54Staining and Imaging
  • 04:08Results: Representative Results of Lactate Dehydrogenase Staining
  • 05:43Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种用于映射酶活性的空间分布的协议, 它能直接在组织样品中生成 nicotinatmide 腺嘌呤二核苷酸磷酸 (NAD (P) h) + h。

Tags

Lactate DehydrogenaseEnzyme ActivityCryomoldCryosectioningHistochemical StainingNADPMSNitrotetrazolium Blue ChlorideLactateEnzyme Localization

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