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JoVE Journal Bioengineering
Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
 

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Article DOI: 10.3791/57763-v • 06:51 min • August 10th, 2018
August 10th, 2018 •
*1, *1, 2, 2, 2, 1, 3, 2, 1
1Department of Bioengineering, The University of Texas at Arlington, 2Department of Medicine (Cardiology) and Bioengineering, UCLA, 3College of Health Science and Environmental Engineering, Shenzhen Technology University
* These authors contributed equally

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Summary
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Here, we present a protocol to visualize developing hearts in zebrafish in 4-Dimensions (4-D). 4-D imaging, via light-sheet fluorescence microscopy (LSFM), takes 3-Dimensional (3-D) images over time, to reconstruct developing hearts. We show qualitatively and quantitatively that shear stress activates endocardial Notch signaling during chamber development, which promotes cardiac trabeculation.

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Keywords: Light-sheet Fluorescence Microscopy 4D Imaging Zebrafish Heart Cardiac Trabeculation Notch Signaling Computational Algorithm Congenital Cardiac Diseases 3D Imaging Agarose Gel Image Processing
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