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用抗体阵列对信号转导通路的审问对酪氨酸激酶抑制剂的抗性评价

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Cancer Research

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JoVE Journal Cancer Research

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Please note that all translations are automatically generated. Click here for the English version.

用抗体阵列对信号转导通路的审问对酪氨酸激酶抑制剂的抗性评价

DOI:

10.3791/57779-v

•

07:42 min

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September 19, 2018

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Katrin Tiemann, Carolina Garri, Jeffrey Wang, Lauren Clarke, Kian Kani

¹Lawrence J. Ellison Institute for Transformative Medicine, Center for Applied Molecular Medicine,University of Southern California

Chapters

00:04Title
00:47Protein Extraction
02:07Human Phosphokinase Array and Data Analysis.
06:03Results: Representative Tyrosine Kinase Inhibitor (TKI) Resistance Analysis
06:48Conclusion

Summary

Automatic Translation

在这里, 我们提出了抗体阵列的协议, 以确定各种细胞模型中信号通路的变化。这些变化, 由药物/缺氧/紫外线光/辐射, 或过度表达/下调/挖空, 是重要的各种疾病模型, 并可以表明治疗是否有效或能识别药物的机制电阻。

Tags

Keywords: Tyrosine Kinase Inhibitors Signal Transduction Pathways Antibody Arrays Phosphorylation Cancer Growth Drug Resistance Therapy Post-translational Modifications Glycosylation Ubiquitylation Cell Lysis Protein Quantification Phosphokinase Assay Array Buffer

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