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Etkili arınma ve on-Eleven Translocation-2 5-metilsitozin Dioxygenase için tahlil LC-MS/MS tabanlı geliştirme
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
DOI:

10:33 min

October 15, 2018

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Chapters

  • 00:04Title
  • 00:45Cloning and Purification of Untagged Human TET2 Dioxygenase
  • 05:305mC Oxidation by TET2 Dioxygenase
  • 07:02Quantitative Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-based Assay Development
  • 08:31Results: Investigating the Role of the TET2 Enzyme in Normal Development and Disease
  • 09:26Conclusion

Summary

Automatic Translation

Burada, on-on translocation-2 (TET2) iletişim kuralı etkin etiketsiz insan bir verimli tek adım arıtma için mevcut iyon değiştirme Kromatografi ve sıvı kromatografi-tandem mass kullanarak onun tahlil kullanarak 5-metilsitozin dioxygenase spektrometresi (LC-MS/MS)-yaklaşım alarak.

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