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Genetics

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JoVE Journal Genetics
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
 

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

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Summary
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Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

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Keywords: Single-cell Analysis Stem Cell Heterogeneity Cancer Heterogeneity Immunophenotypic Profiling Gene Expression Analysis Lineage Potential FACS Sorting Single-cell PCR Combinatorial Approach
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