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組合せの単一セルは分子を特徴付ける方法・肺浸潤異質性ひと幹と前駆細胞集団
JoVE Journal
Genetics
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JoVE Journal Genetics
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
DOI:

09:34 min

October 25, 2018

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Chapters

  • 00:04Title
  • 00:50Lysis Plate Preparation and Cell Sorting
  • 03:35Reverse Transcription and Specific Target Amplification
  • 04:51Preparation of Sample and Assay Plates
  • 07:41Results: Dynamic Characterization of Cell Populations
  • 08:43Conclusion

Summary

Automatic Translation

一括遺伝子発現測定クラウド個々 のセル違い異種細胞集団で。ここでは、プロトコルについて述べる方法単一細胞遺伝子発現解析とインデックスの不均一性の線引きによって蛍光活性化セルの並べ替え (FACS) を並べ替えを結合でき、immunophenotypically 分子細胞集団を特徴付けます。

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