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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
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Biochemistry
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JoVE Journal Biochemistry
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

DOI:
10.3791/57881-v

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05:52 min

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June 28, 2018

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, ,
1Institute of Biochemistry,Graz University of Technology

Chapters

  • 00:04Title
  • 00:43Expression of Modified E. coli Strains
  • 01:41Expression of Bacterial Bioluminescent Strain
  • 02:27In Vivo Activity Assay for Bacterial Bioluminescent Strains and Modified E. coli Strains
  • 03:49Results: Cell Density and Light Emission of lux Operon in E. coli
  • 05:27Conclusion

Summary

Automatic Translation

Automatic Translation

Bioluminescent bacteria regulate light production through a variety of mechanisms, such as quorum sensing. This novel method allows the in situ investigation of bioluminescence and the correlation of light emission to cell density. An artificial bioluminescent Escherichia coli system allows the characterization of lux operons, Lux proteins, and their interplay.

Tags

Keywords: BioluminescenceCell DensityLight EmissionLux OperonBacterial StrainsOvernight CultureIPTG InductionOptical DensityIncubator ShakerArtificial Seawater Medium

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