Journal
/
Genetics
/
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

DOI:
10.3791/57889-v

•

11:22 min

•

August 26, 2018

•

, , , , ,
1Department of Genetics and Genome Sciences,Case Western Reserve University, 2Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center,Case Western Reserve University, 4Center for RNA Science and Therapeutics,Case Western Reserve University

Chapters

  • 00:04Title
  • 01:32Sample Pretreatment
  • 05:00In Situ Hybridization Assay
  • 09:14Results: Use of RNA In Situ Hybridization Assay to Analyze Alternative Pre-mRNA Splicing in Mouse Brain Sections
  • 10:39Conclusion

Summary

Automatic Translation

Automatic Translation

An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.

Tags

Alternative Pre-mRNA SplicingRNA In Situ HybridizationMouse Brain SectionsQuantitative AnalysisIsoform-specific DetectionExon InclusionNF1 Exon 23aSlide PreparationHybridization Assay

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

An Assay for Quantifying Protein-RNA Binding in Bacteria

Read Article