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Journal
/
Genetics
/
RNA
原位
杂交法定量分析小鼠脑切片中的替代前 mRNA 拼接方法
JoVE Journal
Genetics
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JoVE Journal
Genetics
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA
In Situ
Hybridization Assay
Please note that all translations are automatically generated.
Click here for the English version.
RNA
原位
杂交法定量分析小鼠脑切片中的替代前 mRNA 拼接方法
DOI:
10.3791/57889-v
•
11:22 min
•
August 26, 2018
•
Xuan Guo
2
,
Yiqing Zhao
3
,
Hieu Nguyen
,
Tonghua Liu
,
Zhenghe Wang
3
,
Hua Lou
3,4
1
Department of Genetics and Genome Sciences
,
Case Western Reserve University
,
2
Department of Endocrinology
,
Dongfang Hospital of Beijing University of Chinese Medicine
,
3
Case Comprehensive Cancer Center
,
Case Western Reserve University
,
4
Center for RNA Science and Therapeutics
,
Case Western Reserve University
Chapters
00:04
Title
01:32
Sample Pretreatment
05:00
In Situ
Hybridization Assay
09:14
Results: Use of RNA
In Situ
Hybridization Assay to Analyze Alternative Pre-mRNA Splicing in Mouse Brain Sections
10:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种利用短反义寡核苷酸检测小鼠脑部替代前 mRNA 拼接模式的
原位
杂交协议。
Tags
Alternative Pre-mRNA Splicing
RNA In Situ Hybridization
Mouse Brain Sections
Quantitative Analysis
Isoform-specific Detection
Exon Inclusion
NF1 Exon 23a
Slide Preparation
Hybridization Assay
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