Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Behavior

A subscription to JoVE is required to view this content. Sign in or start your free trial.

A Microphysiologic Platform for Human Fat
 

A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

Article DOI: 10.3791/57909-v 08:14 min August 15th, 2018
August 15th, 2018

Chapters

Summary

White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.

Tags

Keywords: Microphysiologic Platform Human Fat Sandwiched White Adipose Tissue Adipose Biology Adipose Stromal Support Cells ASC PNIPAAm Gelatin Solution Seeding Apparatus HBSS Sodium Hydroxide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter