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Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System
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Cancer Research
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JoVE Journal Cancer Research
Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

DOI:
10.3791/57926-v

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11:06 min

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July 30, 2018

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, , ,
1Department of Surgery (RMH),University of Melbourne

Chapters

  • 00:04Title
  • 00:53Dual-adenovirus Transduction in Live Single Cells
  • 02:32Live Single Cell Signaling in Wound Healing Assay
  • 04:16Cell Preparation for Live Signaling Imaging In Vivo
  • 06:49Orthotopic Implantation
  • 08:28Results: Live Single Cell Imaging In Vitro and Live TGF-β Signaling Imaging In Vivo
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for live cell imaging of TGF-β/Smad3 signaling activity using an adenovirus reporter system. This system tracks transcriptional activity in real-time and can be applied to both single cells in vitro and in live animalmodels.

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Keywords: Live Cell ImagingTGF-β/Smad3 Signaling PathwayAdenovirus Reporter SystemCancer Cell LinesBreast CancerWound Healing AssayMDA-MB-231 CellsAdenovirus InfectionFluorescence MicroscopyGFPTd-TomatoTGF-betaImageJ

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