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鼻电位差量化小鼠跨上皮离子转运

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Biology

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JoVE Journal Biology

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

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鼻电位差量化小鼠跨上皮离子转运

DOI:

10.3791/57934-v

•

08:55 min

•

July 04, 2018

•

Mathilde Beka, Teresinha Leal

¹Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC),Université Catholique de Louvain

Chapters

00:04Title
00:49Pre-test Assessment and Management
01:59Assessment and Management During the Test
06:55Results: Representative Nasal PD Tracings
08:03Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个测量小鼠鼻电位差异的协议。该试验量化了囊性纤维化跨膜电导调节剂和上皮钠通道等跨膜离子转运蛋白的功能。评价新疗法对囊性纤维化的疗效有重要意义。

Tags

Nasal Potential Difference Cystic Fibrosis Transgenic Mice Ion Transport Double Lumen Nasal Catheter Electrolyte Cream Voltmeter Peristaltic Pump Data Capture Software

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