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Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
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Chapters
- 00:01Title
- 01:17Growth of Parasites
- 02:53DNA Extraction and PCR Analysis
- 04:22Southern Hybridization
- 04:58Immunological Assays – Immunofluorescence
- 06:22Immunological Assays – ELISA
- 07:16Assessment of the Immune Tolerance
- 08:22Infection of Mice with the Trypanosoma cruzi Chagas Patient’s Semen Ejaculates
- 09:00Representative Results
- 11:54Conclusion
The Trypanosoma cruzi agent of Chagas disease produces long-lasting asymptomatic infections that abruptly develop into clinically recognized pathology. The following research protocol describes a short-run family-based epidemiological study to unravel the T. cruzi infection transmitted sexually from parent to progeny.
