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JoVE Journal Genetics
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
 

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Article DOI: 10.3791/58024-v • 10:12 min • June 29th, 2018
June 29th, 2018 •
1,2, 1, 1,2, 1,2, 1,2, 1, 1, 2, 2, 1, 1,2
1Children's Hospital of Eastern Ontario Research Institute, 2Department of Biology, University of Ottawa

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Summary
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Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.

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Keywords: DNA Extraction Genotyping Zebrafish Larvae Fin Clipping High-throughput Genetic Models Disease Genetic Epilepsies Mutation Study Dissection Anesthetize Tricaine Micro Pipette Scalpel Filter Paper Melanocytes
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