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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
 

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Article DOI: 10.3791/58033-v • 09:57 min • July 12th, 2018
July 12th, 2018 •
1, 1, 1, 1
1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ

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Summary
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Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.

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Keywords: Microbiome Dynamics Flow Cytometry Microbial Ecology Biotechnology Natural Communities Biogas Community Activated Sludge Cell Fixation Cell Stabilization PBS Formaldehyde Ethanol Sonication OD 700 Permeabilization Buffer
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