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Biología celular cuantitativa de neurodegeneración en Drosophila mediante análisis imparcial de fluorescencia etiquetas proteínas con ImageJ
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1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Chapters
- 00:04Title
- 00:50Brain Dissection and Immunofluorescence Staining
- 02:23Image Acquisition and Region of Interest (ROI) Selection
- 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
- 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
- 08:20Conclusion
Hemos desarrollado un flujo de trabajo sencillo y adaptable para extraer datos cuantitativos de estudios biológicos celulares basado en la proyección de imagen de la fluorescencia de la agregación de la proteína y autofagia flujo en sistema nervioso central de Drosophila modelos de neurodegeneración.
Tags
Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
