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哺乳动物细胞系的厌氧生长与维持
JoVE Journal
Biology
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JoVE Journal Biology
Anaerobic Growth and Maintenance of Mammalian Cell Lines
DOI:

07:15 min

July 21, 2018

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Chapters

  • 00:04Title
  • 01:11Prepare Media for Anaerobic Culture of Various Mammalian Cell Lines
  • 02:14Anoxic Cultivation of Various Mammalian Cell Lines
  • 04:33Assessment of Phenotypic Cell Differentiation by Microscopy of Anaerobically Cultured Cells
  • 05:10Results: Cell Viability for Long-term Cultivation Dependent on Initial Seeding Confluence
  • 05:57Conclusion

Summary

Automatic Translation

在这里, 我们描述了一种新的方法, 使厌氧长期培养已建立的细胞系。测试的最大生存时间是17天。该方法适用于细胞毒性药物的检测和 anoxically 复制细胞的生理学研究。

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