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Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
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Cancer Research
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JoVE Journal Cancer Research
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

DOI:
10.3791/58194-v

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10:09 min

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January 07, 2019

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, ,
1Cancer Blood Disease Institute, Divisions of Experimental Hematology and Cancer Pathology,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:04Title
  • 00:48Isolation of c-Kit+ Cells from Bone Marrow
  • 03:52Transduction
  • 06:43Colony-forming Unit Assays
  • 08:25Results: c-Fos and Dusp1 Therapeutic Target in Leukemia
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe protocols for the genetic and chemical validation of c-Fos and Dusp1 as a drug target in leukemia using in vitro and in vivo genetic and humanized mouse models. This method can be applied to any target for genetic validation and therapeutic development.

Tags

Keywords: C-FosDusp1Oncogene DependenceCancer TherapeuticsLeukemiaSolid TumorsLung CancerBrain TumorsPancreatic TumorsBone MarrowMouseCell IsolationCD117MACSCell SeparationCell Culture

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