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Micropuncture Bowmans plads i mus lettes ved 2 foton mikroskopi
JoVE Journal
Biology
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JoVE Journal Biology
Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy

Micropuncture Bowmans plads i mus lettes ved 2 foton mikroskopi

DOI:
10.3791/58206-v

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07:37 min

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October 11, 2018

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, , , , , ,
1Anesthesiology & Perioperative Medicine,Oregon Health & Science University, 2Environmental and Biological Services Division,Pacific Northwest National Laboratory, 3Operative Care Division,Portland Veterans Affairs Medical Center

Chapters

  • 00:04Title
  • 00:42Kidney Stabilization and Injection
  • 02:35Glomerulus Selection and Bowman s Space Fluid Aspiration
  • 05:41Results: Representative Bowman s Space Micropuncture and Analysis
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer anvendelse af 2-foton mikroskopi til at placere en mikropipette inden for Bowmans urin plads i mus, kombinere 2 grundlagsforskning teknikker af renal fysiologi. Anvendelse af 2-foton mikroskopi overvinder kritiske begrænsninger af konventionelle mikroskopi for micropuncture renal fysiologi undersøgelser.

Tags

MicropunctureBowman’s SpaceTwo-photon MicroscopySingle-nephron PhysiologyGlomerular FiltrationTubular PhysiologyMouse PreparationKidney StabilizationFITC-DextranTarget GlomerulusPipette Positioning

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