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A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window
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A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

DOI:
10.3791/678-v

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06:31 min

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February 25, 2008

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1Department of Neurology,University of California, Los Angeles

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:26Tail Vein Injections of Fluorescent Dextran Dyes
  • 02:23Imaging of Blood Flow
  • 05:07Visualization of Blood Flow with In Vivo Two-Photon Imaging
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

Cortical blood flow dynamics can be studied in vivo by imaging fluorescent dextran dyes injected into the tail vein of rodents with 2-photon microscopy. This video shows how to image blood flow dynamics in neocortex of mice through a glass-covered cranial window preparation.

Tags

2-photon ImagingBlood FlowNeocortexCranial WindowCerebral VasculatureLiving RodentsIn Vivo MicroscopyFluorescent DyesCortical VasculatureBlood Flow DynamicsCerebral IschemiaNeurodegenerative DisordersBrain TumorsNormal Brain PhysiologyStrokeRed Blood Cell VelocityFluxInfarctNeocortex Imaging

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