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下一代 16S rRNA 扩增子测序的蜱菌群鉴定

JoVE Journal
Biology

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JoVE Journal Biology

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

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下一代 16S rRNA 扩增子测序的蜱菌群鉴定

DOI:

10.3791/58239-v

•

07:21 min

•

August 25, 2018

•

Lisa Couper, Andrea Swei

¹Department of Biology,San Francisco State University

Chapters

00:04Title
00:44Tick Surface Sterilization, DNA Purification, and 16S rRNA Gene Amplification
02:3016S Amplicon Purification
03:59Sample Barcoding and Purification
04:58Library Quantification and Normalization
06:01Results: Community Ecology of the Ixodes Microbiome
06:43Conclusion

Summary

Automatic Translation

在这里, 我们提出了下一代测序协议 16S rRNA 测序, 使识别和鉴定微生物群落在载体内。该方法包括 DNA 的提取、扩增和条形码样品的 PCR、序列化和生物信息学, 将序列数据与系统进化信息相匹配。

Tags

Tick Microbiome Next-generation 16S RRNA Amplicon Sequencing Vector-borne Disease Ecology Microbiome Characterization Tick Collection DNA Extraction Amplicon PCR Contamination Control Amplification Bias

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