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Isolation and Decellularization of a Whole Porcine Pancreas
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isolation and Decellularization of a Whole Porcine Pancreas

Isolation and Decellularization of a Whole Porcine Pancreas

DOI:
10.3791/58302-v

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09:08 min

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October 10, 2018

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, , ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, 2The Transplant Institute,Sahlgrenska University Hospital

Chapters

  • 00:04Title
  • 01:01Porcine Pancreas Dissection
  • 04:51Decellularization Preparation
  • 06:00Porcine Pancreas Decellularization
  • 07:33Results: Representative Gross Morphology and H&E Staining of Normal and Decellularized Pancreata
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

Tags

Keyword Extraction: Porcine PancreasDecellularizationDissectionBlood VesselsPeristaltic PumpOrgan ChamberDetergent ContainerDetergent Collection ContainerHeparinizedFemale PigMidline IncisionSplenic LobeDuodenal LobeConnection LobeMajor Duodenal PapillaInferior EsophagusSmall IntestineColon

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