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Induktion af aterosklerotiske Plaques gennem aktivering af Mineralocorticoid receptorer i Apolipoprotein E-mangelfuld mus

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JoVE Journal Medicine

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Induktion af aterosklerotiske Plaques gennem aktivering af Mineralocorticoid receptorer i Apolipoprotein E-mangelfuld mus

DOI:

10.3791/58303-v

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07:36 min

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September 26, 2018

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Vincenzo Marzolla, Andrea Armani, Caterina Mammi, Alessandra Feraco, Massimiliano Caprio²

¹Laboratory of Cardiovascular Endocrinology,IRCCS San Raffaele Pisana, Rome, Italy, ²Department of Human Sciences and Promotion of the Quality of Life,San Raffaele Roma Open University, Rome, Italy

Chapters

00:04Title
00:45Osmotic Pump Filling and Implantation
02:38Heart Dissection
04:03Aortic Root Sectioning and Oil Red O Neutral Fat Staining
05:47Results: Evaluation of the Aortic Root Phenotype in Aldosterone-induced Atherosclerosis in Apolipoprotein-E Knockout (ApoE ^-/-) Mice
06:28Conclusion

Summary

Automatic Translation

Vi beskriver en protokol for at fremkalde åreforkalkning i aorta roden af ApoE^{- / -} mus fodret med en atherogene kost, gennem en kontinuerlig frigivelse af aldosteron. Metoder til at karakterisere plaque sammensætning er også beskrevet.

Tags

Atherosclerosis Apolipoprotein E-deficient Mice Mineralocorticoid Receptors Aldosterone Mini-pump Implantation Atherosclerotic Plaque Induction Perfusion

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