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Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin
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Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

DOI:
10.3791/57642-v

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07:38 min

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May 06, 2018

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,
1Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry,University Hospital Tübingen, 2Institute of Diabetes Research and Metabolic Diseases (IDM) of the Helmholtz Center Munich,University Tübingen, 3German Center for Diabetes Research (DZD),University Tübingen

Chapters

  • 00:04Title
  • 00:54Inducing Experimental Nephrotic Syndrome with Doxorubicin
  • 02:24Implantation of Sustained-Release Pellets Containing Aprotinin
  • 04:01Assessing Urine Serine Protease Activity
  • 05:32Results: Analysis of Doxorubicin-induced Nephrotic Syndrome
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the induction of experimental nephrotic syndrome in 129S1/SvImJ mice by rapid retrobulbar injection of doxorubicin. We also treat nephrotic mice with sustained release pellets containing aprotinin to inhibit urinary serine protease activity and prevent sodium retention.

Tags

Nephrotic SyndromeDoxorubicinRetrobulbar InjectionAprotininProteasuriaSodium RetentionEdemaChronic Kidney DiseaseMouse ModelExperimental ProtocolSurgical Procedure

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