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1Neurobiology Laboratory,National Institute of Environmental Health Sciences, 2Comparative Medicine Branch,National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory,National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory,National Institute of Environmental Health Sciences
Chapters
- 00:04Title
- 00:55Mouse Fertilized Egg Isolation
- 02:12Mouse Fertilized Eggs XYClone Laser Perforation
- 03:13Mouse Fertilized Egg Transduction and Non-surgical Transfer
- 04:00Results: Representative Lentivirus Transduction Analyses
- 04:55Conclusion
Ratón huevos fertilizados y embriones de etapa temprana están protegidos por la zona pelúcida, una matriz de la glicoproteína que forma una barrera contra la entrega del gene. Este artículo describe un protocolo para perforar la zona con un láser para transducir las células embrionarias con vectores lentivirales y crear ratones transgénicos.
