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通过成人角质层可视化果蝇腿运动神经元轴突

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Developmental Biology

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JoVE Journal Developmental Biology

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Please note that all translations are automatically generated. Click here for the English version.

通过成人角质层可视化果蝇腿运动神经元轴突

DOI:

10.3791/58365-v

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08:33 min

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October 30, 2018

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Wenyue Guan, Lalanti Venkatasubramanian, Myungin Baek, Richard S. Mann, Jonathan Enriquez

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, ³Neuroscience Program,NYU School of Medicine

Chapters

00:04Title
00:29Leg Dissection and Fixation
02:01Leg Mounting
03:01Imaging and Post Imaging Processing
07:08Results: Representative Drosophila Leg Motor Neuron Axon Imaging
07:43Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个协议, 通过固定, 安装, 成像和后成像步骤, 可视化的荧光灯蛋白在果蝇成人腿的轴突靶向。

Tags

Keywords: Drosophila Leg Motor Neuron Axons Adult Cuticle Fluorescent Proteins Axon Visualization 3D Reconstruction Carbon Dioxide Anesthesia Ethanol Non-ionic Detergent Paraformaldehyde Glycerol Mounting Medium Microscope Slide Coverslip 488 Nm Laser

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