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mub40肽在中性炎症事件检测中的应用
JoVE Journal
Biology
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JoVE Journal Biology
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
DOI:

06:48 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:34Visualization of Neutrophil Activation with Reverse-Inverso-MUB40-Cy5 Peptide
  • 05:16Results: RI-MUB40 Staining Increases Over Time as More Neutrophils Become Activated
  • 06:03Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以检测中性粒细胞的存在在固定/渗透组织学切片, 并评估活纯化中性粒细胞的激活状态。特别是, mub40肽结合了存在于中性粒细胞特异性和三级颗粒中的乳铁蛋白。颗粒内容物通过渗透或中性粒细胞活化暴露, 可以标记中性粒细胞。

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