JoVE Journal
Medicine
Medicine
This content is Open Access.
Chapters
Summary
The fundamentals of radiation planning and delivery for proton therapy using prostate cancer as a model are presented. The application of these principles to other selected disease sites highlights how proton radiotherapy may enhance clinical outcomes for cancer patients.
Tags
Keywords: Proton Therapy Radiation Therapy External Beam Radiation Therapy Solid Tumor Malignancies Cancer Treatment Tumor Localization Patient Immobilization CT Simulation Prostate Cancer
PLAYLIST
Basic Concepts in Cancer Research
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Education • Core: BiologyCancer -
Education • Cell BiologyAn Introduction to Cell Division -
Education • Core: BiologyEpigenetic Regulation -
Education • Cell BiologyAn Introduction to Cell Motility and Migration -
Education • Cell BiologyAn Introduction to Cell Metabolism -
Research • BiologyUsing Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module -
Research • Developmental BiologyInduction and Validation of Cellular Senescence in Primary Human Cells
Cell-Based Assays
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Research • Cancer ResearchCell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry -
Research • Cancer ResearchReal Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells -
Research • BiochemistryAssay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells -
Research • Cancer ResearchCounting Proteins in Single Cells with Addressable Droplet Microarrays -
Research • Cancer ResearchSubcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking -
Research • Cancer ResearchLive Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System -
Research • Cancer ResearchA Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication -
Research • Developmental BiologyInduction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Capturing Cancer Stem Cells
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Research • Cancer ResearchEstablishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma -
Research • Cancer ResearchObtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors -
Research • Cancer ResearchIsolation of Stem-like Cells from 3-Dimensional Spheroid Cultures -
Research • Cancer ResearchIsolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples -
Research • BiologyIsolation of CD133+ Liver Stem Cells for Clonal Expansion -
Research • Cancer ResearchRadiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Circulating Tumor Cells and DNA
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Research • Cancer ResearchCharacterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH -
Research • Cancer ResearchSemi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence -
Research • Cancer ResearchMicromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment -
Research • Immunology and InfectionAnalyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow -
Research • Cancer ResearchDetection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids -
Research • Cancer ResearchDetection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
Mapping Cancer in Spheroids and 3D Culture
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Research • MedicineChick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds -
Research • BioengineeringPreparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates -
Research • Cancer ResearchMultidimensional Coculture System to Model Lung Squamous Carcinoma Progression -
Research • Cancer ResearchMonitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture -
Research • Cancer ResearchAssessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells -
Research • MedicineImaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids -
Research • Cancer ResearchAnalyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System -
Research • Cancer ResearchHow to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases -
Research • Cancer ResearchLongitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Research on Animal Models
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Research • Cancer ResearchA Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research -
Research • MedicineDual Bioluminescence Imaging of Tumor Progression and Angiogenesis -
Research • MedicineAn Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy -
Research • Cancer ResearchImmunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry -
Research • Cancer ResearchAn Orthotopic Resectional Mouse Model of Pancreatic Cancer -
Research • Cancer ResearchUtilizing High Resolution Ultrasound to Monitor Tumor Onset and Growth in Genetically Engineered Pancreatic Cancer Models -
Research • MedicineMurine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence -
Research • Cancer ResearchUsing the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers
Cancer Histology and Pathology
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Research • MedicineDissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling -
Research • MedicineInstillation and Fixation Methods Useful in Mouse Lung Cancer Research -
Research • MedicineDIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens -
Research • Cancer ResearchPerfusion and Inflation of the Mouse Lung for Tumor Histology -
Research • Cancer ResearchElastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue -
Research • MedicineTubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection -
Research • Cancer ResearchUtilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
Tracking Tumor Metastases
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Research • MedicineExperimental Metastasis Assay -
Research • MedicineModels of Bone Metastasis -
Research • Cancer ResearchCombined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs -
Research • Cancer ResearchAdvanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones -
Research • Cancer ResearchAcellular and Cellular Lung Model to Study Tumor Metastasis -
Research • Cancer ResearchA Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer -
Research • Cancer ResearchPathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells -
Research • MedicineQuantitative Analysis of Cancer Metastasis using an Avian Embryo Model -
Research • MedicineGene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Oncogene Profiling
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Research • Cancer ResearchUsing CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells -
Research • BiologyDetecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing -
Research • Cancer ResearchMapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis -
Research • MedicineQuantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors -
Research • MedicineMicroarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer -
Research • Cancer ResearchSequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients -
Research • MedicineProfiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells -
Research • MedicineIdentification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
Investigating Therapies
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Research • MedicineCerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring -
Research • Cancer ResearchThe Clinical Application of Tumor Treating Fields Therapy in Glioblastoma -
Research • Cancer ResearchAnticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles -
Research • MedicineProton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies -
Research • ChemistryPolymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine? -
Research • MedicineProtocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy -
Research • Cancer ResearchPreparation of Exosomes for siRNA Delivery to Cancer Cells -
Research • Cancer ResearchAnalysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
