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Journal
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Biochemistry
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生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用
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Biochemistry
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JoVE Journal
Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Please note that all translations are automatically generated.
Click here for the English version.
生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用
DOI:
10.3791/58393-v
•
12:25 min
•
September 28, 2018
•
Anthony Asher
,
Iniyan Ganesan
,
Laura Klasek
,
Steven M. Theg
1
Department of Plant Biology
,
University of California – Davis
Chapters
00:04
Title
00:30
Chloroplast Isolation and Quantification
03:30
Isolation of Thylakoids
04:42
Stromal Extract (SE) Recovery
06:33
Transport through the cpTat Pathway
07:44
Transport through the cpSec1 and cpSRP Pathways
08:59
Thermolysin Treatment
10:16
Results: Transport of Proteins Through cpTat and cpSec1 and Integration Through cpSRP Pathways
11:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文提出了用于叶绿体双精氨酸易位 (cpTat)、分泌 (cpSec1) 和信号识别粒子 (cpSRP) 通路的生理活性类囊体和蛋白质转运测定的高产量隔离的协议。
Tags
Thylakoids
Protein Transport
Chloroplast Membranes
Protein Targeting
Percoll Gradient
Chloroplast Isolation
Pea Shoots
Homogenization
Centrifugation
Chlorophyll Concentration
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