JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
原发性白血病细胞代谢谱的评价
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Assessment of the Metabolic Profile of Primary Leukemia Cells
Please note that all translations are automatically generated.
Click here for the English version.
原发性白血病细胞代谢谱的评价
DOI:
10.3791/58426-v
•
06:21 min
•
November 21, 2018
•
Katerina Hlozková
,
Julia Starková
1
CLIP – Childhood Leukemia Investigation Prague, Second Faculty of Medicine, Laboratory of Molecular Genetics
,
Charles University, Prague
Chapters
00:04
Title
00:28
Bone Marrow Mononuclear Cell Isolation and Culture
01:33
Extracellular Flux Analysis Preparation
02:41
Cell Seeding and Sensor Cartridge Loading
04:11
Results: Representative Leukemic Blast Extracellular Flux Analysis
05:54
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们提出了一个方案, 从白血病患者骨髓中分离白血病细胞, 并分析他们的代谢状态。对原代白血病细胞代谢状况的评估有助于更好地确定原代细胞的需求, 并可导致更个性化的药物。
Tags
Primary Leukemia Cells
Metabolic Profile
Metabolic Pathway
Extracellular Flux Analysis
Density Gradient Centrifugation
Mononuclear Cells
Cell Adhesive
Extracellular Flux Analyzer
RPMI Medium
Cell Counting
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/58426?article_type=v
3维球形共培养胰腺癌细胞和成纤维细胞的制备及代谢试验
check_url/58426?article_type=v
离心淘洗制备不同细胞周期的原发性急性淋巴细胞白血病细胞
check_url/58426?article_type=v
流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访
check_url/58426?article_type=v
初级毛质素黑色素细胞和纤维细胞培养的快速生成
check_url/58426?article_type=v
木尿造血干细胞和祖细胞和MLL-AF9驱动白血病线粒体反应氧物种的流细胞学分析
check_url/58426?article_type=v
循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估
check_url/58426?article_type=v
原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运
check_url/58426?article_type=v
NKG2D 利甘德表面检测的两种流细胞学方法,以区分干细胞与急性骨髓性白血病中的散装亚群
check_url/58426?article_type=v
从原代细胞和培养细胞制备细胞质和核长RNA
check_url/58426?article_type=v
小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因
Read Article