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原发性白血病细胞代谢谱的评价
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JoVE Journal Cancer Research
Assessment of the Metabolic Profile of Primary Leukemia Cells
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原发性白血病细胞代谢谱的评价

DOI:
10.3791/58426-v

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06:21 min

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November 21, 2018

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1CLIP – Childhood Leukemia Investigation Prague, Second Faculty of Medicine, Laboratory of Molecular Genetics,Charles University, Prague

Chapters

  • 00:04Title
  • 00:28Bone Marrow Mononuclear Cell Isolation and Culture
  • 01:33Extracellular Flux Analysis Preparation
  • 02:41Cell Seeding and Sensor Cartridge Loading
  • 04:11Results: Representative Leukemic Blast Extracellular Flux Analysis
  • 05:54Conclusion

Summary

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Automatic Translation

在这里, 我们提出了一个方案, 从白血病患者骨髓中分离白血病细胞, 并分析他们的代谢状态。对原代白血病细胞代谢状况的评估有助于更好地确定原代细胞的需求, 并可导致更个性化的药物。

Tags

Primary Leukemia CellsMetabolic ProfileMetabolic PathwayExtracellular Flux AnalysisDensity Gradient CentrifugationMononuclear CellsCell AdhesiveExtracellular Flux AnalyzerRPMI MediumCell Counting

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