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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

DOI:
10.3791/58448-v

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11:45 min

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January 07, 2019

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,
1Department of Cellular and Integrative Physiology,University of Nebraska Medical Center, 2Department of Anesthesiology,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:44Isolation of Cardiac Stem Cells (CSCs)
  • 07:08Characterization of Cardiac Stem Cells
  • 07:54Differentiation of Cardiac Stem Cells into Cardiomyocytes
  • 08:46Results: Successful Isolation of the CSCs and Their Differentiation Toward Cardiomyocytes
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

Tags

Keywords: Cardiac Stem CellsCSCsAdult Mouse HeartIsolationCharacterizationDifferentiationCardiovascular DiseaseHeart FailureCardiac Stem Cell TherapyCost-effectiveHam’s Balanced Salt SolutionCollagenase IICell Suspension

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