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成人小鼠心脏干细胞的分离、鉴定及分化
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JoVE Journal Biology
Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
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成人小鼠心脏干细胞的分离、鉴定及分化

DOI:
10.3791/58448-v

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11:45 min

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January 07, 2019

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,
1Department of Cellular and Integrative Physiology,University of Nebraska Medical Center, 2Department of Anesthesiology,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:44Isolation of Cardiac Stem Cells (CSCs)
  • 07:08Characterization of Cardiac Stem Cells
  • 07:54Differentiation of Cardiac Stem Cells into Cardiomyocytes
  • 08:46Results: Successful Isolation of the CSCs and Their Differentiation Toward Cardiomyocytes
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

本文的总体目标是规范心脏干细胞 (csc) 与成年小鼠心脏分离、表征和分化的协议。在这里, 我们描述了一种密度梯度离心方法, 以分离小鼠 csc 和详细的方法, csc 培养, 增殖, 并分化为心肌细胞。

Tags

Cardiac Stem CellsCSCsAdult Mouse HeartIsolationCharacterizationDifferentiationCardiovascular DiseaseHeart FailureCardiac Stem Cell TherapyCost-effectiveHam’s Balanced Salt SolutionCollagenase IICell Suspension

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