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JoVE Journal Neuroscience
Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
 

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Article DOI: 10.3791/58503-v • 11:01 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 1
1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health

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Summary
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Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.

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Keywords: Quantitative Autoradiographic Method Regional Rates Cerebral Protein Synthesis In Vivo Brain Protein Synthesis Quantitative Awake Behaving Animal Autoradiographic Technique Femoral Artery Femoral Vein Surgical Procedure Suture Catheter Heparinized Saline
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