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Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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Chapters
- 00:04Title
- 00:43Femoral Vein and Artery Catheterization
- 04:46Tracer Administration and Collection of Blood Samples
- 06:07Analysis of Plasma Samples to Determine Concentrations of Leucine and L-[1-14C]leucine
- 07:07Quantitative Autoradiography
- 07:58Image Analysis and Computation of Regional Rates of Cerebral Protein Synthesis (rCPS)
- 09:02Results: Effects of Anisomycin on Rates of Cerebral Protein Synthesis
- 09:57Conclusion
Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.
