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小鼠血睾丸屏障完整性的一种活体研究方法
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Developmental Biology
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JoVE Journal Developmental Biology
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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小鼠血睾丸屏障完整性的一种活体研究方法

DOI:
10.3791/58512-v

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05:48 min

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December 02, 2018

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, , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Chapters

  • 00:04Title
  • 00:25Inulin-FITC Administration
  • 02:20Tissue Harvest and Imaging
  • 04:00Results: Representative Blood-Testis Barrier (BTB) Integrity Assessment
  • 04:50Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议, 通过在睾丸中注入 inulin-fitc 来评估血液睾丸屏障的完整性。这是一种有效的体内方法, 研究血液睾丸屏障完整性, 可由遗传和环境元素损害。

Tags

Blood-testis BarrierIn Vivo MethodMouseCadmium ChlorideTestisInulin-FITCMicroinjectionCryosectioningParaformaldehydeOptimal Cutting Temperature Compound

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