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Journal
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Developmental Biology
/
小鼠血睾丸屏障完整性的
一种活体
研究方法
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
An
In Vivo
Method to Study Mouse Blood-Testis Barrier Integrity
Please note that all translations are automatically generated.
Click here for the English version.
小鼠血睾丸屏障完整性的
一种活体
研究方法
DOI:
10.3791/58512-v
•
05:48 min
•
December 02, 2018
•
Mengrou Liu*
1
,
Chunsen Zhu*
1
,
Shun Bai*
2
,
Xin Li
,
Kaiqiang Fu
,
Lan Ye
,
Ke Zheng
1
State Key Laboratory of Reproductive Medicine
,
Nanjing Medical University
,
2
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine
,
University of Science and Technology of China
Chapters
00:04
Title
00:25
Inulin-FITC Administration
02:20
Tissue Harvest and Imaging
04:00
Results: Representative Blood-Testis Barrier (BTB) Integrity Assessment
04:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 通过在睾丸中注入 inulin-fitc 来评估血液睾丸屏障的完整性。这是一种有效
的体内
方法, 研究血液睾丸屏障完整性, 可由遗传和环境元素损害。
Tags
Blood-testis Barrier
In Vivo Method
Mouse
Cadmium Chloride
Testis
Inulin-FITC
Microinjection
Cryosectioning
Paraformaldehyde
Optimal Cutting Temperature Compound
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