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Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
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Developmental Biology
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JoVE Journal Developmental Biology
Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

DOI:
10.3791/58524-v

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07:25 min

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March 20, 2019

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,
1Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza),Centro de Investigación y Tecnología Agroalimentaria de Aragón

Chapters

  • 00:04Title
  • 00:46Dormancy Determination and Plant Material Collection
  • 01:49Plant Material Preparation for Starch Quantification
  • 03:07Quantification of the Starch Content
  • 05:10Results: Flower Bud Growth and Dormancy Evaluated after 10 Days in Suitable Conditions
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

We present a methodology to quantify the starch content in the ovary primordia in sweet cherry (Prunus avium L.) during winter dormancy by using an image analysis system combined with histochemical techniques.

Tags

Starch QuantificationOvary PrimordiaSweet CherryWinter DormancyHistochemical StainingImage AnalysisFlower BudsDormancyReproductive PhaseLugol’s Iodine StainingBrightfield MicroscopyImage Acquisition

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