JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we describe a method for purifying histidine-tagged pyrophosphokinase enzymes and utilizing thin layer chromatography of radiolabelled substrates and products to assay for the enzymatic activity in vitro. The enzyme activity assay is broadly applicable to any kinase, nucleotide cyclase, or phosphor-transfer reaction whose mechanism includes nucleotide triphosphate hydrolysis.
