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Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
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1Department of Hematology,Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery,Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine,Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences,Wuhan University, 5Department of Gastroenterology,Central Hospital of Wuhan, 6Department of Transfusion,Zhongnan Hospital of Wuhan University
Chapters
- 00:04Title
- 00:34Immunohistochemistry (IHC) Lung Tissue Sample Preparation
- 03:56Automatic Whole-Slide Scanning
- 04:52Slide Image Analysis
- 05:50Results: Representative IHC Staining in Lung Cancer Tissue Samples
- 06:27Conclusion
ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.
