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Developmental Biology

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JoVE Journal Developmental Biology
Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
 

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

Article DOI: 10.3791/58587-v • 10:45 min • June 3rd, 2019
June 3rd, 2019 •
1,2, *1,2, *1,2, 1,2, 1,2
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden
* These authors contributed equally

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Summary
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This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

Tags

Keywords: Actomyosin Dynamics Drosophila Egg Chambers Time-lapse Movies Cell Segmentation Epithelial Cells Surface Manager Fiji Image Analysis Cell Membrane Cell Outline
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