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1Department of Pathology,University of Cambridge, 2Department of Physiology, Development and Neuroscience,University of Cambridge, 3Department of Obstetrics and Gynaecology,University of Cambridge School of Medicine, NIHR Cambridge Biomedical Research Centre, 4Centre for Trophoblast Research,University of Cambridge, 5Department of Genetics & Evolution,University of Geneva, 6Royal Papworth Hospital, 7Department of Plant Sciences,University of Cambridge
Chapters
- 00:04Title
- 01:45Preparation and Plating Out of DNA
- 02:25Preparation of the Master Mix
- 03:36qPCR Assay
- 04:07Post-run Analysis
- 05:19Export of Results and Copy Number Calculations
- 06:39Results: qKAT Used to Copy Number Type KIR Genes
- 06:59Conclusion
Quantitative des cellules tueuses récepteur immunoglobuline-like (KIR) saisie semi-automatique (qKAT) est une méthode simple, haut-débit et rentable pour copier le numéros gènes de type KIR pour leur application dans les études d’association de la population et de la maladie.
Tags
Keywords:
QKATQuantitative KIR Automated TypingKiller-cell Immunoglobulin-like ReceptorNK Cell Receptor GeneticsHaplotype DiversityDisease AssociationHigh-throughputGene Copy NumberMultiplex PCRReal-time PCRRelative QuantificationPopulation GeneticsHLAViral InfectionsCancerStem Cell TransplantationPregnancy Disorders
