/
/
Paramyxovirus for Tumor-målrettet Immunomodulation: Design og evaluering Ex Vivo
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:47Generation of Recombinant Virus Particles
- 02:47Collection and Propagation of Virus Particles
- 05:11Viral Titer Evaluation
- 06:59Determining Replicative and Cytotoxic Capacities of Viral Vectors Encoding Immunomodulators
- 08:03Assessment of Immunological Functionality of Isolated Virus-encoded Immunomodulators from Infected Cells
- 10:00Results: Evaluation of Tumor-targeted Immunomodulation by Paramyxoviruses
- 11:37Conclusion
Denne protokol beskriver en detaljeret arbejdsgang for generation og ex vivo karakterisering af oncolytic virus for udtryk for hæmmende, ved hjælp af mæslinger virus kodning bispecific T-celle ager som eksempel. Ansøgning og tilpasning til andre vektor platforme og transgener vil fremskynde udviklingen af nye immunovirotherapeutics for klinisk oversættelse.
