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Journal / Genetics / Isolating, Sequencing and Analyzing Extracellular …
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Genetics

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JoVE Journal Genetics
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
 

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Article DOI: 10.3791/58655-v • 10:55 min • March 8th, 2019
March 8th, 2019 •
*1, *1, 1,2, 1, 1, 1,2
1The Interdisciplinary Nanoscience Centre, Aarhus University, 2Department of Molecular Biology and Genetics, Aarhus University
* These authors contributed equally

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Summary
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This protocol demonstrates how to purify extracellular microRNAs from cell culture media for small RNA library construction and next generation sequencing. Various quality control checkpoints are described to allow readers to understand what to expect when working with low input samples like exRNAs.

Tags

Keywords: Extracellular MicroRNAs Mesenchymal Stem Cells Next-generation Sequencing Extracellular Vesicles RNA Isolation Nanoparticle Tracking Analysis Ultracentrifugation Quality Control
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