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Journal / Genetics / LINE-1 Methylation Analysis in Mesenchymal Stem…
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JoVE Journal Genetics
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
 

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Article DOI: 10.3791/60705-v • 12:18 min • February 1st, 2020
February 1st, 2020 •
*1,2, *1,2, 1,2, 1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital
* These authors contributed equally

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Summary
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Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.

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Keywords: LINE-1 Methylation Mesenchymal Stem Cells Osteosarcoma Extracellular Vesicles DNA Methylation Epigenetics Ultracentrifugation EV Isolation FBS Depletion
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