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1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Chapters
- 00:04Title
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
यहां, हम polyacrylamide जैल में कुल प्रोटीन का पता लगाने के लिए एक नया फ्लोरोसेंट धुंधला तकनीक रूपरेखा एक विस्तृत प्रोटोकॉल का वर्णन । प्रोटोकॉल एक चांदी आयन विशिष्ट प्रतिदीप्ति बारी पर जांच, जो एजी का पता लगाता है का उपयोग करता है+-प्रोटीन परिसरों, और पारंपरिक chromogenic चांदी के दाग की कुछ सीमाएं समाप्त ।
